Nitrofuran (SEM) ELISA Test Kit
1. Principle
This test kit is based on the competitive enzyme immunoassay for the detection of semicarbazide
(SEM) in chicken, fish, shrimp, milk, honey, whole egg. The coupling antigens are pre-coated on
the micro-well stripes. The SEM in the sample and the coupling antigens pre-coated on the microwell
stripes compete for the anti-SEM antibodies. After the addition of the enzyme conjugate, the
TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative
correlation with the SEM in it. This value is compared to the standard curve and the SEM
concentration is subsequently obtained.