Nitrofuran (AOZ) ELISA Test Kit

Catalog No. LSY-10002

1. Principle

This test kit is based on the competitiveenzyme immunoassay for the detection of AOZ in the sample. Thecoupling antigens are pre-coated onthe micro-well stripes. The AOZ in thesample and the couplingantigens pre-coated on the micro-well stripes compete for the anti-AOZ antibodies.After the addition of the enzyme conjugate, the TMB substrate is added forcoloration. The optical density (OD) value of the sample has a negative correlationwith the AOZ in it. This value is compared to the standard curve and the AOZ concentrationis subsequently obtained.

2. Technical specifications

Sensitivity:  0.01ppb

Incubation Temperature:  25℃

Incubation Time:  30min～15min

Cross-reaction rate

AOZ................................................................................................ 100%

AMOZ............................................................................................ <0.1%

AHD.............................................................................................. <0.1%

SEM.............................................................................................. <0.1%

Recovery rate

Tissue.......................................................................................... 95±25%

Honey.......................................................................................... 85±23%

3. Components

1）    Micro-wellstrips: 12 strips with 8 removable wells each

2）    6× standard solution(1 mL each): 0ppb, 0.01ppb, 0.03ppb, 0.09ppb, 0.27ppb and 0.81ppb

3）    Enzymeconjugate (7 mL).............................................................. redcap

4）    Antibodyworking solution (7 mL)................................................. bluecap

5）    Substrate Asolution (7 mL)........................................................ whitecap

6）    Substrate Bsolution (7 mL)........................................................ blackcap

7）    Stop solution(7 mL)................................................................. yellowcap

8）    20×concentrated washing buffer (40 mL).................................... whitecap

9）    2×concentrated redissolving solution (50 mL).................... transparentcap

10）  2-Nitrobenzaldehyde (C₇H₅NO₃)(10mL)........................................ whitecap