Tylosin ELISA Test Kit

1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Tylosin in the sample. The coupling antigens are pre-coated on the micro-well stripes. The Tylosin in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Tylosin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Tylosin in it. This value is compared to the standard curve and the Tylosin concentration is subsequently obtained.

2. Technical specifications

Sensitivity: 1.5 ppb

Detection limit

Meat, liver........................................................................................ 3 ppb

Honey........................................................................................... 1.5 ppb

Recovery rate

Meat............................................................................................. 90±15%

Liver............................................................................................. 80±10%

Honey.......................................................................................... 85±15%

3. Components

1)   Micro-well strips: 12 strips with 8 removable wells each

2)   6× standard solution (1 mL each): 0 ppb, 1.5 ppb, 4.5 ppb, 13.5p pb ,40.5 ppb,121.5 ppb

3)   Enzyme conjugate (7 mL)............................................................... red cap

4)   Antibody working solution (7 mL).................................................. blue cap

5)   Substrate A solution (7 mL).......................................................... white cap

6)   Substrate B solution (7 mL)......................................................... black cap

7)   Stop solution (7 mL).................................................................. yellow cap

8)   20× concentrated washing buffer (40 mL)..................................... white cap

9)   2× concentrated redissolving solution (50 mL)..................... transparent cap