Enrofloxacin ELISA Test Kit

Catalog No. LSY-10017

1. Principle

This test kit is based on the competitive enzyme immunoassay. The coupling antigens is pre-coated on the micro-well stripes. The enrofloxacin residue in the sample and coupling antigens pre-coated on the micro-well stripes compete for the anti-enrofloxacin antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the content of enrofloxacin residue in it. This value is compared to the standard curve and the content of the corresponding Enrofloxacin residue is subsequently obtained.

2. Technical specifications

Sensitivity : 0.25 ppb

Detection limit:

Tissue........................................................................................... 0.5 ppb

Serum........................................................................................... 0.5 ppb

Honey............................................................................................. 1 ppb

Cross-reaction rate:

Enrofloxacin..................................................................................... 100%

Ciprofloxacin................................................................................... 1.25%

Norfloxacin..................................................................................... 1.65%

Other Xacin medicines..................................................................... <0.1%

Recovery rate:

Tissue.......................................................................................... 85±15%

Serum.......................................................................................... 80±15%

Honey.......................................................................................... 75±15%

3. Components

1)   Micro-well strips: 12 strips with 8 removable wells each

2)   6 × standard solution (1 mL each): 0 ppb, 0.25 ppb, 0.75 ppb, 2.25 ppb, 6.75 ppb and 20.25 ppb

3)   Enzyme conjugate (7 mL)............................................................... red cap

4)   Antibody working solution (7 mL).................................................. blue cap

5)   Substrate A solution (7 mL).......................................................... white cap

6)   Substrate B solution (7 mL)......................................................... black cap

7)   Stop solution (7 mL).................................................................. yellow cap

8)   20× concentrated washing buffer (40 mL)..................................... white cap

9)   2× concentrated redissolving solution (50 mL)..................... transparent cap