Gentamicin ELISA Test Kit

Catalog No. LSY-10023

1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Gentamicin in the sample. The coupling antigen is pre-coated on the micro-well stripes. The Gentamicin in the sample and pre-coated coupling antigen on the micro-well stripes compete for the anti-Gentamicin antibody. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Gentamicin in it. The value is compared to the standard curve and the Gentamicin concentration is subsequently obtained.

2. Technical specifications

Sensitivity: 0.1 ppb

Incubator temperature:  25℃

Incubator time:  60min～15min

Detection limit

Chicken, Liver................................................................................... 4 ppb

Cross-reaction rate

Gentamicin........................................................................................ 100%

Streptomycin...................................................................................... <1%

Dihydrostreptomycin........................................................................... <1%

Neomycin........................................................................................... <1%

Recovery rate

Chicken, Liver................................................................................ 70±18%

3. Components

1）   Micro-well strips: 12 strips with 8 removable wells each

2）   6× standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb, 8.1 ppb

3）   Enzyme conjugate (7 mL)............................................................... red cap

4）   Antibody working solution (7 mL).................................................. blue cap

5）   Substrate A solution (7 mL).......................................................... white cap

6）   Substrate B solution (7 mL)......................................................... black cap

7）   Stop solution (7 mL).................................................................. yellow cap

8）   20× concentrated washing buffer (40 mL)..................................... white cap

9）   2× concentrated redissolving solution (50 mL)..................... transparent cap