Nitrofuran (AMOZ) ELISA Test Kit Catalog No. LSY-10001 1. Principle This test kit is based on the competitive enzyme immunoassay for the detection of AMOZ in the sample. The coupling antigens are pre-coated on the micro-well stripes. The AMOZ in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-AMOZ antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the AMOZ in it. This value is compared to the standard curve and the AMOZ concentration is subsequently obtained. 2. Technical specifications Sensitivity: 0.1 ppb Detection limit Tissue (Chicken, duck, porcine meat, beef and mutton) 0.2 ppb Liver (Chicken, duck, porcine meat, beef and mutton) 0.2 ppb Honey, milk, intestine, urine 0.2 ppb Fish, shrimp (some interference) 0.3 ppb Recovery rate Tissue, liver 90±15% Honey, milk, intestine 80±15% Cross-reaction rate AMOZ 100% AHD <0.01% AOZ <0.01% SEM <0.01% 3. Components 1) Micro-well strips: 12 strips with 8 removable wells each 2) 6× standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb and 8.1 ppb 3) Enzyme conjugate (7 mL) red cap 4) Antibody working solution (7 mL) blue cap 5) Substrate A solution (7 mL) white cap 6) Substrate B solution (7 mL) black cap 7) Stop solution (7 mL) yellow cap 8) 20× concentrated washing buffer (40 mL) white cap 9) 2× concentrated redissolving solution (50 mL) transparent cap 10) 2-Nitrobenzaldehyde (C7H5NO3)(10 mL) white cap