AmpicillinELISA Test Kit

Catalog No. LSY-10018

1. Principle

Thistest kit is based on the competitive enzyme immunoassay for the detection of Ampicillinin samples. The antigens conjugated Ampicillin ispre-coated on the micro-well stripes, Ampicillin in the sampleand the conjugated antigens pre-coated on the micro-well stripes compete forthe anti-Ampicillin antibodies. After the addition of the enzyme conjugate, theTMB substrate is added for coloration. The optical density (OD) value of thesample has a negative correlation with Ampicillinconcentration in the sample. This value is compared to the standard curve andconcentration of Ampicillin in the sample issubsequently obtained.

2. Technical specifications

Sensitivity: 0.1 ppb

Incubator temperature:  25℃

Incubator time:  30min~30min~15min

Detection limit:

Tissue, honey, milk ........................................................................... 2ppb

Because of some interference, quantificationlimit of honey, milk sample is 4 ppb

Cross-reaction rate:

Ampicillin........................................................................................... 100%

Benzyl penicillin.................................................................................. 0.8%

Cloxacillin........................................................................................... 0.2%

Dicloxacillin........................................................................................ 0.1%

Amoxicillin.......................................................................................... 0.1%

Ceftiofu.............................................................................................. 0.1%

Recovery rate:

Tissue............................................................................................ 75±19%

Honey, milk.................................................................................... 70±17%