Clenbuterol ELISA Test Kit

Catalog No. LSY-10012

1. Principle

This test kit is based on the competitive enzyme immunoassay for the detection of Clenbuterol in urine, tissue etc. The coupling antigen is pre-coated on the micro-well stripes. The Clenbuterol in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-Clenbuterol antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the Clenbuterol in the sample. This value is compared to the standard curve and the Clenbuterol residues is subsequently obtained.

2. Technical specifications

Sensitivity: 0.1 ppb

Incubation Temperature: 25℃

Incubation Time: 30min—15min

Detection limit

Urine............................................................................................ 0.1 ppb

Tissue (method one)..................................................................... 0.4 ppb

Tissue (method two)...................................................................... 0.1 ppb

Feed............................................................................................... 1 ppb

Recovery rate

Urine............................................................................................ 95±22%

Tissue.......................................................................................... 75±20%

Feed............................................................................................ 80±20%

Cross-reaction rate

Clenbuterol...................................................................................... 100%

Terbutalin.......................................................................................... <1%

Mabuterol ........................................................................................ <1%

Brombuterol...................................................................................... <1%

Salbutamal....................................................................................... < 1%

Ractopamine.................................................................................... < 1%

3. Components

1)   Micro-well strips: 12 strips with 8 removable wells each

2)   6× standard solution (1 mL each): 0 ppb, 0.1 ppb, 0.3 ppb, 0.9 ppb, 2.7 ppb ,8.1 ppb

3)   Enzyme conjugate (7 mL)............................................................... red cap

4)   Antibody working solution (10 mL)................................................ blue cap

5)   Substrate A solution (7 mL)......................................................... white cap

6)   Substrate B solution (7 mL)......................................................... black cap

7)   Stop solution (7 mL).................................................................. yellow cap

8) 20X Sample extract solution (50 mL)…………………………………. blue cap