Aconite water-soluble total alkaloid has anti-heart failure, anti-shock and other effects, the main component is alkaloids, followed by alcoholamines, lipids and polysaccharides. The macroporous adsorption resin is used as an adsorbent to extract and purify the water-soluble total alkaloid from the aconite by physical adsorption. The method has the advantages of simple equipment, easy operation and high product purity. Therefore, the macroporous resin adsorption method is increasingly widely used in the extraction and purification of water-soluble total alkaloids from aconite.