Lamiophlomis rotata is one kind of the commonly used herbal medicines. It is mainly used for a variety of surgical operations such as knife pain, bleeding, traumatic fractures and other symptoms. The chemical components that have been obtained in the lamiophlomis rotata are mainly flavonoids, anthraquinones and saponins. The study found that the total flavonoids in the lamiophlomis rotata have obvious analgesic effect. Using macroporous adsorption resin to extract flavonoids from lamiophlomis rotata has the advantages of simple equipment, easy operation and high product purity, and has good application prospects.