Berberine is a quaternary ammonium alkaloid isolated from the traditional Chinese medicine Coptis, which is the main active ingredient of Coptis. Widely used in the treatment of gastroenteritis, bacterial dysentery, etc., acute tonsillitis and respiratory infections also have a certain effect. The macroporous adsorption resin is used as an adsorbent to adsorb berberine from the aqueous solution by physical adsorption. The method has the advantages of simple equipment, easy operation and high product purity. Therefore, the macroporous resin adsorption method is increasingly widely used in the purification and purification of berberine.