In vitro diagnostic HBsAg Elisa Kit
USD $5.5 - $7.86 /Box
Min.Order:1 Box
HBsAg Elisa Kit
HBsAg Elisa Kit is Enzyme-linked immunosorbent assay for the detection of HBsAg in serum or plasma
INTRODUCTION |
HBsAg ELISA kit is used for the qualitative determination of Hepatitis B surface antigen (HBsAg) in human serum or plasma. This test is indicated for the screening of blood and blood products to be used for transfusion and an aid for the diagnosis of existing or previous hepatitis B infection.
PRINCIPLE OF THE ASSAY |
HBsAg ELISA kit is a solid-phase simultaneous sandwich immunoassay, which employs monoclonal antibodies and polyclonal antibodies specific for HBsAg. Microtiter well is coated with monoclonal antibodies specific for HBsAg. A serum specimen is added to the antibody coated Microtiter wells together with enzyme conjugated polyclonal antibodies. HBsAg, if present, will form an antibody-HBsAg-antibody-enzyme complex. The plate is then washed to remove unbound material. Finally, a solution of substrate is added to the wells and incubated. A blue color will develop in proportion to the amount of HBsAg present in the specimen. The enzyme-substrate reaction can be stopped and the result is visualized by naked eye or read by EIA plate reader for absorbance at the wavelength of 450 nm(450 nm/630 nm) and is proportional to the amount of antibodies present in the specimen..
SPECIMEN COLLECTION AND PREPARATION |
Serum should be prepared from a whole blood specimen obtained by acceptable medical techniques. Either serum or plasma can be used in this test. Remove serum or plasma from the clot or blood cells as soon as possible to avoid hemolysis. Specimen with extensive particulate should be clarified by centrifugation prior to use. Specimen frozen at -20°C or colder may be used. Avoid repeated freeze thaw.
PRECAUTIONS FOR USERS |
STORAGE OF TEST KITS |
Unopened test kits should be stored at 2-8°C. DO NOT FREEZE KIT COMPONNETS. The microtiter plate should be kept in a sealed bag to minimize exposure to damp air. Use up the reagents as soon as possible after the kit is unpacked.
Our Services
1:Factory direct price .price is competitive with high quality.
2: Original manufacturer provide OEM service for you.
3: Smooth delivery near beijing and tianjin Port.
4: Free samples available sent for your evalutaiton.
5: ISO 9001,ISO13485,GMP,Medical devices-quality management system certificate
Company Information
Our company is in Beijing, China beginning built in 1988.Specialized in the field of ELISA TESTS&RAPID TESTS as both supplier and manufacturer with GMP,ISO9001:2008, SFDA and ISO13485 certificates. Our company was the earliest domestic high-tech enterprise which specializes in the research, test, manufacture and sale of genetic engineering drugs and diagnostic reagent.
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