Product Details

Specifications

1. Free charge of sample
2. Easy operation
3. Low price
4. High sensitivity and high quality
5. OEM package is available

Company Profile

Beijing Huan Biotechnology Co., Ltd. BRIC in 2005 by Beijing Sihuan Pharmaceutical Co., Ltd. [listed company, Jiangsu Sihuan Bioengineering Co., a subsidiary] from discrete reorganization, was originally a subsidiary company of Military Medical Sciences; beginning built in 1988, is the earliest in genetic engineering drugs and diagnostics research, test, production and sales of high-tech enterprises.

name keyword : EBV ELISA reagent
2005 in Beijing Daxing Industrial Development Zone Overseas Students Pioneer Park built a 1500 square meters of in vitro diagnostic reagents standard production workshop, 2008 has passed GMP certification, the Certificate of GMP, and through ELISA, gold standard quality management system assessment. In 2009 the company obtained the ISO9001: 2008 quality management system certification. All work to achieve standardized management.

The company has research and development department, quality control department, production department, marketing department, comprehensive department, logistics, warehousing, Personnel Administration Department, General Manager's Office, etc.; business managers experienced, proficient in the medical profession; company established through the introduction and cultivation of talents of a high-quality management, research and development, engineering, quality control, production, marketing and administrative professionals, formed a strict and comprehensive production quality management system.

Enterprises and Chinese Military Academy of Medical Sciences signed a long-term technical cooperation agreement with the Chinese Academy of Sciences and other domestic research institutes to establish a close contact. Employ a number of medicine, biology became a biomedical expert committee of experts, relying on these forces and our unremitting efforts, the company will continue to introduce new bio-medicine, service to society.

PRINCIPLE OF THE ASSAY

The EBV-VCA IgA Ab ELISA Kit utilizes a detection system where micro plate wells are coated with recombinant antigen corresponding to a highly antigenic segment EBV-VCA. Serum or plasma specimens, controls are added to the wells. During incubation, IgA antibodies specific for EBV-VCA present in the specimen will bind to the recombinant antigen fixed onto the micro plate wells. The wells are washed to remove unbound materials, and anti-human IgA peroxides conjugate will bind to the antigen-antibody complex and excess unbound enzyme conjugates are again removed by washing. The enzyme substrate, tetramethylbenzidine (TMB), is added upon incubation the substrate will be hydrolyzed by the bound enzyme and a blue or blue-green color develops in wells containing EBV-VCA IgA antibodies. The enzyme reaction is stopped by the addition of sulphuricacid. The intensity of color developed is read spectrophotometrically at 450nm(450 nm/630 nm) and is proportional to the amount of antibodies present in the specimen.

RESULT INTERPRETATION

EIA Reader at 450 nm (using the OD value of the blank well to correct all the OD reading from all wells or at 450 nm/630 nm with dual filter plate reader.

Cut-off Calculations:

0.10 + average OD values of Negative control:

0.10 +NC x = Cut-off.

If the absorbance of negative controls is below 0.05, calculate it as 0.05.If the absorbance of negative controls is above 0.05, calculate it as its original value.