Specifications

1. Free charge of sample
2. Easy operation
3. Low price
4. High sensitivity and high quality
5. OEM package is available

Enzyme-linked immunosorbent assay for the detection HBeAg in serum or plasma

INTENDED USE

 

HBeAg EIA is a qualitative enzyme immunoassay for the detection of HBeAg in human serum or plasma.

 name: medical diagnostic kit blood reagents HBe Ag   keyword:  blood reagents

SUMMARY AND PRINCIPLE OF THE TEST

 

The Hepatitis B e antigen is found to be related to Hepatitis B viral infection. HBeAg is usually found in the early phase of Hepatitis B viral infection. The titer of HBeAg rises rapidly during the period of virus replication. The presence of HBeAg correlates with increasing numbers of infectious viruses (Dane particles) and the presence of viral specific DNA polymerase in serum. During the HBeAg positive stage, hepatitis B patients are at increasing risk of transmitting the virus to their contacts. Persistent presence of HBeAg in the hepatitis B virus carrier is often associated with chronic active hepatitis.

 

The HBeAg EIA is a sandwich immunoassay, which employs monoclonal and polyclonal antibodies specific for HBeAg. Microtiter wells are coated with monoclonal antibodies specific for HBeAg. A serum specimen is added to the antibody coated microtiter wells together with enzyme conjugated polyclonal antibodies. HBeAg, if present, will form an antibody-HBeAg-antibody-enzyme complex. The plate is then washed to remove unbound materials. Finally, a solution of substrate is added to the wells and incubated. A blue color will develop in proportion to the amount of HBeAg present in the specimen. The enzyme substrate reaction can be stopped and the result is visualized by the naked eye or obtained by EIA plate reader for absorbance at wavelength of 450 nm (450 nm/630 nm).

 

REAGENTS

 

Materials provided with the kits:

1. Microtiter well coated with monoclonal anti-HBe: 96 tests in one bag.
2. Negative Control: 0.5ml HBeAg Negative Control.
3. Positive Control: 0.5ml HBeAg Positive Control.
4. Enzyme Conjugate:.6 ml containing HRP-conjugated-anti-HBe
5. Wash Buffer Concentrate (20 x): 20ml for 96 tests. The buffer should be diluted 20 times with distilled water before use.
6. Substrate Solution A: 6 ml HRP Substrate for 96 tests.
7. Substrate Solution B: 6 ml TMB Chromagen Substrate for 96 tests.
8. Stop Solution: One bottle of 6ml 2N Sulfuric Acid

Materials required but not provided:

1. Precision pipettes: 0.02, 0.05, 0.10, 0.15, 0.20, and 1.0 ml.
2. Disposable pipette tips.
3. Distilled water.
4. HumidifiedBox capable of maintaining 37°C
5. Absorbent paper or paper towel.
6. Micro titer plate or strip-well washer
7. 

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