Specifications

1. Free charge of sample
2. Easy operation
3. Low price
4. High sensitivity and high quality
5. OEM package is available

INTRODUCTION

 

Hepatitis C virus (HCV), which was formerly described as the parent rally transmitted form of non-A, non-B hepatitis (NANBH)¹, becomes a chronic disease in 50% of the cases.²   HCV can also be transmitted through intravenous drug abuse, sexual , and household contact.³ Hepatitis C virus is a single stranded RNA virus with some structural relations to the flavivirus family. Nucleic acid sequences of HCV cDNA clones provided the basis for the construction of recombinant peptides representing putative hepatitis C virus proteins.^4,5Anti-hepatitis C virus antibody screening of blood using synthetic or recombinant proteins, helped to identify apparently healthy blood donors with anti-HCV antibodies who otherwise might have transmitted the virus.⁶

This is an enzyme linked immunosorbent assay using recombinant proteins derived from core regions of HCV virus to detect the presence of HCV antibodies in human sera.

 

Materials provided with the kits:

Item	Description	96T
1	Coating Plate	1
2	Negative Control	1ml
3	Positive Control	1ml
4	Sample Diluents	12ml
5	Enzyme Conjugate	12ml
6	Wash Buffer Concentrate (20x)	30ml
7	Substrate Solution A	6ml
8	Substrate Solution B	6ml
9	Stop Solution	6ml
10	Plastic Bag	1
11	Seal Paper	3
12	Manual	1 copy

 

Materials required but not provided:

1. Precision pipettes: 0.02, 0.05, 0.10, 0.15, 0.20, and 1.0 ml.
2. Disposable pipette tips.
3. Distilled water.
4. HumidifiedBox capable of maintaining 37°C
5. Absorbent paper or paper towel.
6. Micro titer plate or strip-well washer
7. Micro titer plate reader.

   PRECAUTION FOR USERS

   All human source material used in the preparation of this product was found to be negative for the presence of HCV antibodies, as well as for the hepatitis B surface antigen, using a commercial licensed method. Nevertheless, because no test method can offer complete assurance of the absence of infectious agents, this product should be handled with caution. Avoid contact of reagents with the eyes and skin. If that occurs, wash thoroughly with water.

   1. Wear gloves.
   2. Do not pipette by mouth.
   3. Do not smoke.
   4. Dispose all used materials in a suitable biohazard us waste container. Remains of samples, controls, aspirated reagents and pipette tips should be collected in a container for this purpose and autoclaved 1-hour at 121°C or treated with 10% sodium hypochlorite (final concentration) for 30 min before disposal. (Remains containing acid must be neutralized prior addition of sodium hypochlorite).
   5. Adjust washer to the plate used (flat bottom) in order to wash properly.
   6. Do not mix reagents from different lots.
   7. Do not use reagents after expiration date.
   8. Extreme care should be taken to avoid microbial contamination and cross contamination of reagents.
   9. Use a new pipette tip for each specimen and each reagent.
   10. Soaps and/or oxidizing agents remaining in containers used for the substrate-TMB solution can interfere with the reaction.
   11. 
   12. Sales department

   13. Aileen
       Bioneovan Co.,Ltd 
       Skype: aileen.li2