Tetracycline Rapid Test (Honey)

Cat No.: W81063-1

1.        INTENDED USE

Quicking Tetracycline Rapid Test is a competitive immunoassay for the semi-quantitative detection of the presence of Tetracycline residue in honey sample.

Cut-off:   20 ppb

Assay Time:  10 - 15 min

2.        PRINCIPLE OF THE ASSAY

Quicking Tetracycline Rapid Test is based on competitive lateral flow immunochromatographic assay. The Tetracycline conjugate in the test zone will capture the immuno-gold (colloid gold- Tetracycline antibody conjugate), when there is very little dissociative Tetracycline in the samples. A visible red test band indicates a negative result when the control line (C zone) shows that the card is valid. The test band (T zone) will be not visible if Tetracycline is present in concentration of 20 ppb and above which explains a positive result.

3.        KIT COMPONENT

-           10×foil pouches each containing one cassette and a desiccant

-           10×assay buffer (0.75 mL each) 

-           20×pipettes

-           1×plastic canister contained 10 microwells and a desiccant

-           Products Manual

4.        TEST PROCEDURE

-           Add 0.25 mL of honey sample into an assay buffer tube and mix well. If there is crystal, thaw the sample in a water bath (60-80°C) beforehand.

-           Take out the microwells strip from the plastic canister. Take one well and tear off the film.

-           Suck 0.2 mL of the extract into the well with another pipette (the reticle level is 0.2 mL content). Repeatedly suck and extrude the sample until all red reagents are completely dissolved. Wait for 1 min.

-           Take out the cassette from the foil pouch and place it horizontally.

-           Suck the mixture in the well and gradually drop 3 drops into the assay sample hole “S”.

-           Interpret the result in 10-15 min. Result after 15 min is considered as invalid.

5.        INTERPRETATION OF RESULTS

Positive: Only one clear band in C zone C indicates a positive result. If a vague T band can be seen but apparently weaker than C band, we also consider it as a positive result. Positive shows that the concentration of Tetracycline is above 20 ppb (ng/mL) in the sample.

Negative: The presence of both clear bands in C zone and T zone. (T band is close to or stronger than C band.)

Invalid: No colored band appears in C zone.

6.        SPECIFICITY

The results are negative when the test card is applied to detecting 100 ppm (μg/mL) of Chloramphenicol, Aminoglycosides, Beta-lactams, Sulfonamides, and Macrolides.

7.        STORAGE

The kit can be stored at room temperature (2-30°C). The test kit is stable through the expiration date (18 months) marked on the package label. DO NOT FREEZE. Do not store the test kit in direct sunlight.