Quinolone Rapid Test (Honey)

Cat No.:  W81074-1

1. INTENDED USE

Quicking Quinolone Rapid Test is a competitive immunoassay for the semi-quantitative detection of the presence of Quinolone residue in honey sample.

Cut-off: 20 ppb

Assay Time: 5- 10 min

2. PRINCIPLE OF THE ASSAY
Quicking Quinolone Rapid Test is based on competitive lateral flow immunochromatographic assay. The QLN-conjugate in the test zone will capture the immuno-gold (colloid gold-QLN antibody conjugate), when there is very little dissociative QLN in the samples. A visible red test band indicates a negative result when the control line (C zone) shows that the card is valid. The test band (T zone) will be not visible if QLN is present in concentration of 20 ppb and above which explains a positive result.

3. KIT COMPONENT
- 10× foil pouches each containing a cassette and desiccant
- 1 × plastic canister containing 10 microwells and a desiccant
- 2× assay buffer (Diluent A, 30 mL, Diluent B, 8 mL)
- 2× centrifugal tubes (50 mL)
- 1 0× pipettes
- Product Manual

4. TEST PROCEDURE
- Add 4.0 g of honey sample into a 50 mL centrifugal tube. Add 2 mL of diluent A and shake for a good dissolution.. If there is crystal, thaw the sample in a water bath (60-80℃ ) beforehand.
- Add 3 mL of Ethyl acetate and 6 mL of Dichloroethane. Shake for 5 min. Stand the tube until the lower layer gets clear.
- Transfer 5 mL of lower layer into a beaker. Dry the liquid by blowing wind. Redissolve the residues in the small beaker with 300 μ L of Diluent B.
- Take out the microwells strip from the plastic canister. Take one well and tear off the film. Suck 0.2 mL of the extract into the well with the pipette (the reticle level is 0.2 mL content). Repeatedly suck and extrude the sample until all red reagents are completely dissolved. Wait for 1 min.
- Take out the cassette from the foil pouch and place it horizontally.
- Gradually drip 3 drops of sample extraction into the sample hole “S”.
- Interpret the result in 5 - 10 min. Result after 10 min is only considered as reference.

5. INTERPRETATION OF RESULTS

Positive:  Only one clear band in C zone indicates a positive result. Positive shows that the concentration of Quinolone is at or above 20 ppb in the sample.
Negative: The presence of both clear bands in C zone and T zone.
Invalid: No colored band appears in C zone.

6. SPECIFICITY
- The results are negative when the test is applied to detect 100 ppm of Chloramphenicol, Aminoglycosides, Macrolides, beta-lactam and Tetracycline.

7. STORAGE
The kit can be stored at room temperature (2-30°C). The test kit is stable through the expiration date (1 2 months) marked on the foil pouch. DO NOT FREEZE. Do not store the test kit in direct sunlight.

8. PRECAUTIONS

- For best results, please strictly adhere to these instructions.
- All reagents must be at room temperature before running the assay.
- Do not remove test cassette from its pouch until immediately before use.
- Do not reuse the test kit.
- Do not use the test beyond its expiration date marked on the foil pouch.
- The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot
numbers.

9. LIMITATION
Quicking Quinolone Rapid Test is a useful tool offering a rapid and accurate testing in field screening, exceeding with its convenience. It provides a semi-quantitative method to detect the Quinolone above 20 ppb in honey sample. If you want a quantitative result, please adopt other method such as ELISA in practice.