Specifications

The device can accurately indicate the presence of Beta lactam residue in
milk.

  Beta lactam Rapid Test

Cat No.: W81035 

 

1. INTENDED USE
Quicking Beta lactam Rapid Test is a competitive immunoassay for the semi-quantitative detection of the presence of Beta lactam residue in milk.

Assay Time: 5 – 1 0 min

Sample: milk

2. KIT COMPONENT
- 6× plastic canister each containing 8 microwells and 8 strips
- 48× pipettes
- Product Manual

 

3.  TEST PROCEDURE

 

 

- Collect milk as testing sample.
- Take out the microwells and strips of necessary quantity from the plastic canister. Recover the milk and test kit to room temperature (20-25 °C). Do not heat the milk.
- Use pipette to transfer 200 μ L of sample (a mark on the pipette) into the microwell.
- Repeatedly suck and extrude the sample until all red reagents are completely dissolved.
- Stand for 2 – 3 min and insert the “max” end of strip into the well.
- Read the result in 5 - 1 0 min. Result after 1 0 minutes is only considered as reference.

 

4. INTERPRETATION OF RESULTS

 

Positive: Only clear C band appears.
Negative: The presence of both C band and T band.
Invalid: No colored band appears in C zone.

 

5. SENSITIVITY AND SPECIFICITY
- Penicillin G 2 - 4 ppb, Ampicillin 10 - 15 ppb, Amoxicillin 4 - 5 ppb
- Oxacillin 10 - 15 ppb, Cloxacillin 10 - 15 ppb
- Cefacetril 125 ppb, Cefquinome 15 - 25 ppb
- Ceftiofur 50 ppb, Cefapirin 60 ppb.
- The results are negative when the rapid test is applied to detect 100 ppm of Chloramphenicol, Aminoglycosides, Tetracyclines, Macrolides and Sulfonamides.

6. STORAGE
All materials must be stored at 2 - 8°C. The test kit is stable through the expiration date (1 2 months) marked on the foil pouch. Do not store the test kit in direct sunlight.

7. PRECAUTIONS
- For best results, please strictly adhere to these instructions.
- All reagents must be at room temperature before running the assay.
- Do not remove rapid test from its pouch until immediately before use.
- The milk sample should be fresh and homogeneous liquid without caking and precipitation.
- Do not reuse the test strip.
- Do not use the test beyond its expiration date
- Do not use water, powdered milk as negative control. Use confirmed negative fresh milk as control.
- The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot
numbers.

8. LIMITATION
Quicking Beta lactam Rapid Test is an useful tool offering a rapid and accurate testing in field screening, exceeding with its convenience. If you want a quantitative result, it is suggested to apply other method such as ELISA / HPLC in practice.