Streptomycin (SM) ELISA Test Kit
Storage conditionsl The kit shall be stored at [2-8 ℃]. Expiry date: 12 months
l The opened Microelisa Stripplate can be stored at [2-8 ℃] and avoid damp. Use for at least 2 months.
IntroductionStreptomycin (SM) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Streptomycin in honey, tissue, and etc.
Principle of testThe Streptomycin (SM) ELISA Test Kit is based on a competitive colorimetric ELISA assay.
The Streptomycin (SM) coupling antigen has been coated in the plate wells. During the detection, sample is added along with the primary antibody specific for the target drug. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the drug attached to the well. The secondary antibody, tagged with a Horseradish peroxidase enzyme, targets the primary antibody that is complexed to the drug coated on the plate wells. Take coloration with TMB substrate. The samples A value is an inverse relationship with SM residue content. Lastly, compared with the standard curve can be concluded the SM residues in sample.
Technique Datal Kit sensitivity: 0.1 ppb(ng/ml)
l reactive mode: 25℃, 30min～30min～15min
l Detection Limits:
Sample | Detection Limits |
Tissue | 4 ppb |
Honey | 2 ppb |
Milk, Milk powder | 5 ppb |
l Cross-reaction rate:
Name | Cross-reaction rate |
Streptomycin | 100% |
Dihydrostreptomycin | 100% |
Kanamycin | 6.3% |
Gentamicin | 2.5% |