Sulfonamides (SAs) ELISA Test Kit
Storage conditionsl The kit shall be stored at [2-8 ℃]. Expiry date: 12 months
l The opened Microelisa Stripplate can be stored at [2-8 ℃] and avoid damp. Use for at least 2 months.
IntroductionSulfonamides (SAs) ELISA Test Kit is a competitive enzyme immunoassay for the quantitative analysis of Sulfonamides in honey, tissue, serum, milk, urine, and etc.
Principle of TestThe Sulfonamides (SAs) ELISA Test Kit is based on a competitive colorimetric ELISA assay.
The plate wells are coated with capture antibodies directed against anti- sulfonamide antibodies. Sulfonamide standards or sample solution, sulfonamide–HRP conjugate and anti-sulfonamide antibodies are added. Free sulfonamide and sulfonamide-HRP Conjugate compete for the sulfonamide antibody binding sites. At the same time, the anti- sulfonamide antibodies are also bound by the immobilized capture antibodies. Any unbound sulfonamide-HRP Conjugate is then removed in a washing step. Take coloration with TMB substrate. The samples A value is an inverse relationship with SAs residue content. Lastly, compared with the standard curve can be concluded the SAs residues in sample.
Technique Datal Kit sensitivity: 0.5 ppb(ng/ml)
l reactive mode: 25℃, 45min～15min
l Detection Limits:
Sample | Detection Limits |
Tissue ( High detection limit method ) | 0.5 ppb |
Tissue ( Low detection limit method ) | 2.5 ppb |
Urine, Serum | 2 ppb |
Honey | 0.5 ppb |
Milk | 10 ppb |