QPCR(Polymerase Chain Reaction)

QPCR(Polymerase Chain Reaction) gives you a choice of quantitative methods for gene expression.The TL988 Real-time PCR is very useful and important for life science research and curative evaluation, and it is innovative and pioneering products in Lab. for the prevention, diagnosis, evaluation and monitoring of diseases.

Specifications

Profiles	1-9
Repetitions	1-99
Steps	1-9
Set times	1-9999sec
Profile linkage	Yes
Reagents	FAM,SYBR GREEN I,JOE,HEX,VIC (various fluorescence PCR reagents , such as TaqMan probe, etc
Sample capacity	48 × 0.2ml or 36 × 0.2ml
Sample handing	Thick-walled 0.2ml PCR tubes
Sample volume	20-100μl
Wavelength of exciting fluorescence	495nm,535nm
Wavelength of fluorescence detection	525nm,555nm
Monochromator device	Filter,made in USA
Photo detector	PMT(Photomultipier tube), made in Japan
Heating/cooling device	TE Peltier(Thermoelectric Peltier Effect module),made in Japan
Quantitative accuracy	R≥0.990, CV≤3%
Heating rate	3°C/sec
Cooling rate	2°C/sec
Thermal range	4.0°C-99.0°C
Thermal accuracy	±0.5°C
Thermal uniformity	±0.5°C
System interfaces	RS-232C serial interface, bi-directional
Computer operating system	Windows 2000/XP
Power supply	AC220V/110V, 50Hz/60Hz
Power	<800W
Operating environment	Ambient Temperature 5°C-40°C Relative Humidity 30%-70%
Weight	26kg
Dimensions(W×D× H)	500mm × 400mm × 350 mm

Heating & cooling unit

Applying the advantages of thermoelectric (TE, Peltier Effect) module makes temperature change rapidly and uniform. Hot-lid design permits oil-free operation.

Optical unit

An array of LED collimates to corresponding wells.High-light and long-lived LED excitation light source does not need maintenance or preheating.

PMT fluorescence detection offers high sensitivity, broad dynamic range and low crosstalk.

An array of detecting coaxial optical fibers focus on each well’s bottom ensure minimal light loss.

Operation system and Application software

Application software interface is friendly windows style, Monitoring real-time amplification plots and data analysis during a PCR run.

Supply software package of medical case and database management and plot display.

Quantification

After quantitative analysis for the standard sample, the instrument will get a standard curve of Ct value related to copy number.

Comparing the Ct value of unknown sample with standard curve, the instrument can calculate the original copy number of unknown sample by quantitative analysis. TL988 has a wide linear detecting range, which can detect 101-1010 DNA copies/ml.