Cultivation for its spectacular spring flowers is successful in a range of climatic conditions provided summers are not too hot, with trees being grown as far north as Edmonton, Alberta^,the Faroe Islands,and Harstad, Norway. In more southern areas, growth is best in cooler mountain climates.

In Britain and Ireland, the nuts are used for the popular children's game conkers. During the two world wars, horse-chestnuts were used as a source of starch which in turn could be used via the Clostridium acetobutylicum fermentation method devised by Chaim Weizmann to produce acetone. This acetone was then used as a solvent which aided in the process of ballistite extrusion into cordite, which was then used in military armaments.

The nuts, especially those that are young and fresh, are slightly poisonous, containing alkaloid saponins and glucosides. Although not dangerous to touch, they cause sickness when eaten. Some mammals, notably deer, are able to break down the toxins and eat them safely. They are reputed to be good for horses with wind, but this is unproven and feeding them to horses is not advisable. The saponin aescin (a complex mixture of triterpene glycosides), however, has been used for health purposes (such as varicose veins, edema, sprains) and is available in food supplements, as is the coumarin glucoside aesculin.

In the past, horse-chestnut seeds were used in France and Switzerland for whitening hemp, flax, silk and wool. They contain a soapy juice, fit for washing of linens and stuffs, for milling of caps and stockings, etc., and for fulling of cloth. For this, 20 horse-chestnut seeds were sufficient for six liters of water. They were peeled, then rasped or dried, and ground in a malt or other mill. The water must be soft, either rain or river water; hard well water will not work. The nuts are then steeped in cold water, which soon becomes frothy, as with soap, and then turns milky white. The liquid must be stirred well at first, and then, after standing to settle, strained or poured off clear. Linen washed in this liquid, and afterwards rinsed in clear running water, takes on an agreeable light sky-blue colour. It takes spots out of both linen and wool, and never damages or injures the cloth.

In Bavaria the chestnut is the typical tree for a beer garden. Originally they were planted for their deep shade which meant that beer cellar owners could cut ice from local rivers and lakes in winter to cool the Märzen Lager beer well into summer. Nowadays guests enjoy the shade to keep their heads cool - even after the second Maß (a mug with a liter of beer).

Conkers have been threatened by the leaf-mining moth Cameraria ohridella, whose larvae feed on horse chestnut leaves. The moth was described from Macedonia where the species was discovered in 1984 but took 18 years to reach Britain.

Aesculus hippocastanum is used in Bach flower remedies. When the buds are used it is referred to as "Chestnut Bud" and when the flowers are used it is referred to as "White Chestnut".