Anthraquinone saponins are a class of glycosides in which the aglycone is a triterpenoid or a helixane compound, and are mainly present in the mother plant. With antibacterial, antipyretic, sedative, anti-cancer and other effects. The macroporous adsorption resin is used as an adsorbent, and the timosaponin is adsorbed and purified from the aqueous solution by physical adsorption. The method has the advantages of simple equipment, easy operation and high product purity. Therefore, the macroporous resin adsorption method is increasingly widely used in the purification and purification of timosaponin.