Molecular fluorescence spectrophotometer

Instrument Introduction:

This fluorescence spectrophotometer is a high-end molecular fluorescence product independently developed by our company. It enables high-speed three-dimensional scanning and phosphorescence testing. The core components are sourced from renowned international suppliers, ensuring stable performance, powerful functionality, ultra-high signal-to-noise ratio, ultra-fast scanning speed, ultra-high resolution, ultra-high wavelength accuracy, diverse testing content, and abundant testing accessories. It is widely applied in fields such as food safety inspection, materials research, and environmental monitoring, particularly for water quality analysis and control, catering to customers with a broad range of sample tests and stringent performance requirements. It is versatile for both qualitative and quantitative analysis as well as scientific research.

Instrument Features:

• High-Quality Scanning: Ensures precise and accurate spectra with multiple spectral scanning options and an ultra-fast scanning speed of up to 30,000 nm/min. The sophisticated data acquisition system guarantees abundant, complete, and accurate data points.

• Three-Dimensional Scanning: Rapid processing and positioning with leading-edge high-speed digital signal processing technology enable high-quality three-dimensional scanning, significantly enhancing sample analysis, testing, and work efficiency.

• Specially Designed Chopper Mechanism: This innovative and uniquely structured chopper mechanism, in coordination with the control system, achieves high-precision control, ensuring accurate phosphorescence detection. It leverages the long-lived nature of phosphorescence to completely eliminate interference from stray light such as Rayleigh and Raman light, enabling effective constant measurement.

• Phosphorescence Measurement: Capable of measuring phosphorescence lifetimes as short as one millisecond, applicable to various sample types.

• Dual Monochromator System: Equipped with emission shutters and light control devices, significantly improving test results and stability while prolonging product life.

• Versatile Testing Capabilities: Suitable for testing multiple sample types, including fluorescence, chemiluminescence, and phosphorescence, meeting diverse customer needs. Offers multiple test modes, including wavelength scanning, time scanning, velocity scanning, and three-dimensional scanning.

Parameter Configuration:

1. Testing Functions: Fluorescence, bio/chemiluminescence, and phosphorescence lifetime.

2. Testing Categories: Wavelength scanning, time scanning, photometric measurement, and three-dimensional scanning.

3. Light Source: Imported, ozone-free, high-brightness, daylight-colored, with a color temperature close to 6000K, continuous spectrum in the visible region, high color rendering (CRI > 95), maintains light color characteristics throughout its lifetime, high arc stability, hot restart capability, reaches near-full light output immediately after startup, 150W high-intensity, long-life high-pressure xenon lamp.

4. Dispersion Optics: Concave holographic grating, lower stray light, higher resolution (1200L/mm), superior test resolution (better than 1.0 nm), blaze wavelengths: excitation 300 nm, emission 400 nm.

5. Wavelength Range: Zero-order light and 200–760 nm; extendable to 200–900 nm.

6. Sensitivity: Greater than 300:1 water Raman peak (P-P).

7. Wavelength Accuracy: ±0.2 nm.

8. Wavelength Repeatability: ≤ 0.1 nm.

9. Scanning Speeds: 30, 60, 240, 1200, 2400, 12000, 30000 nm/min.

10. Spectral Bandwidths: EX: 1.0, 2.5, 5.0, 10.0, 20.0 nm; EM: 1.0, 2.5, 5.0, 10.0, 20.0 nm.

11. Response Time: 0.004 – 8s (8 adjustable levels, automatic response).

12. Photometric Range: -9999 to 9999.

13. Gain: 4 adjustable levels.

14. PMT Voltage: 1) For controlling photomultiplier tube detector voltage: 250V/400V/700V/950V; 2) Fine-tunable within 0–1000V.

15. Scan Time: 100–18000 ms (in ms); 10–18000 s (in s).

16. Minimum Sample Volume: 0.5 mL (10 mm standard cuvette).

17. Power Consumption: 400mW.

18. Transmission Method: USB interface.

19. Voltage Requirement: 220V/50Hz.

20. Light-Shielding Shutter: Effectively prevents fluorescence decay in tested samples.

21. Gated Shutter: Enhances and prolongs the lifespan of core instrument components.

Software Features:

• Wavelength Scanning: Scans fluorescence excitation and emission spectra of samples.

• Time Scanning: Generates curves showing sample changes over time at fixed excitation and emission wavelengths, calculating phosphorescence or luminescence lifetimes.

• Photometric Measurement: Enables quantitative detection of samples.

• Three-Dimensional Scanning: Simultaneously displays and tracks wavelength and peaks in 2D spectra for easy observation of specific segments.

• Sensitivity Calculation: A popular foreign method, yielding fast and reliable results.

• Spectrum Area Calculation: Computes the area of selected regions in the current spectrum.

• Xenon Lamp Characteristic Spectral Line: Obtained through excitation and emission modes in fluorescence intensity data mode under wavelength scanning speed. Used to calibrate excitation or emission monochromators.

• Dual Background Subtraction: Unique feature resolves inconsistencies in standard sample blanks during testing, improving efficiency.

• Spectral Calibration: Calibrates the instrument's spectral response function to obtain authentic fluorescence spectrum data.

• Water Raman Peak: Obtained through emission mode in wavelength scanning.

• Spectrum Calculation: Performs arithmetic operations (addition, subtraction, multiplication, division) on any two spectra at the same point.

• Automatic Pre-Scan: Quickly identifies spectral information of unknown samples while avoiding misidentification of scattering peaks as fluorescence excitation or emission peaks.

Application Areas:

• Quantum Dot Fluorescence Material Characterization

• Fluorescence Material Lifetime (LED Phosphor, Dashboard Fluorescent Substances) Measurement

• Oil Hydrocarbon Content Detection in Marine Organisms

• Selenium Content Measurement in Food, Feed, Water, and Marine Organisms

• Benzo(a)pyrene Content Measurement in Food and Water

• Aflatoxin Content Measurement in Food

• Vitamin B2 Content Measurement in Food

• Vitamin B1 Content Measurement in Food

• Vitamin C Content Measurement in Infant Food, Dairy Products, Vegetables, Fruits, and Their Products