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Polyamide–High adsorption for Prep Chromatography

Polyamide is widely used for separating botanical extracts (natural products) with phenolic and polyphenolic groups.

The swelling properties enable a high adsorption capacity—much higher than silica gel, alumina, PSDVB, and most other sorbents currently available.

It’s ideally suited for preparative chromatography, but also can is used for analyzing phenolic and polyphenolic substances in natural products.

Polyamide Chromatography Applications:

It’s commonly used to isolate and identify natural substances with phenolic and polyphenolic functional groups such as:

l Flavonoids

l Anthocyanins

l Anthoxanthines

l Anthraquinones

l Isoflavones

l CBD

l Flavones and flavonols

Through modification of adsorption and partition effects, the retention characteristics of polyamide can be varied substantially. Due to the medium polarity of polyamide, the stationary phase (polyamide swollen with eluent) can be used with a mobile phase that is more polar or less polar. It’s possible to develop in two dimensions with normal and reversed-phase chromatography using the same stationary phase.

Adsorption Depends On:

l The number of the polar groups,

l The degree of the H-bonding activities,

l Size of the analytes, and

l The shape of the analytes.

Thus, structural isomers can be well separated on polyamide.

Typical Polyamide Applications:

l Phenols, aromatic nitro, and amino compounds

l Chalcones, chinones, flavones, and anthraquinones

l Anthocyanins and anthoxanthines

l DNP-amino acids

l Carbonic acids and their Amides

l Sulfonic acids and their amides.