Real-time Quantitative PCR Detection System DW-TL988-IV

Design for flexible use

uides you with ease through plate setup.

The DW-TL-988 system adapts to almost any workflow requirement with flexible instrument control and data management.

>> Standard with 7’’ LCD touch screen, functions quickly accessible through simple, one-touch commands

>> High-throughput up to 96 samples per run

>> Push-pull, electrical controlled elevating platform

>> Concealed lid with constant temperatureand isopiesticpressure bring high accurate temperature control

>> Quick-start set-up so you can begin a run immediately

>> Unique power-off protectionfunction can save all the setted configurations for sudden power outages, and allow the experiment continues when power supply is restored.

>> System can be installed in three distinct configurations, providing unmatched flexibility and convenience.  

1. Co-located configuration (PC control)

2. Standalone (PC-free) configuration (Data can be export by USB and analysis on PC)

3. Networked configuration (PC software automatically detect all systems in LAN and allow to remote monitoring one of experimental progress and downloading of the completed run file to the PC at your desk)

Fast and uniform heating and heat sinking block

>> Concealed lid with constant temperatureand isopiesticpressure

>> Electrical controlled elevating & automatic locked platform

>>Thermo block is based on improved heat pipe technology, leading to extraordinary Iintra-well temperature homogeneity and maximized inter-well, inter-cycle reproducibility.

>> Highly efficient PID&DSP control algorithm ensures faster respond and stronger interference resistance, fast and uniform heating and heat sinking

High sensitive and precise optical system

>> High-sensitivity and wide dynamic range of fluorescence detection system, sample dynamic range could be from 10¹ to 10¹⁰ copies

>> Fast and precise mechanical scanning structure ensuresthe illumination uniformity of 96 wells, Eliminate edge effects, no need for ROX calibration

>> Fluorescence excitation by a high-intensity and long-life LED, Bulb Luminance will not attenuation over time, free maintenance optical system through the entire life.

>> Professional designed fluorescence filters realize the highly fluorescence transmittance and meanwhile minimize the fluorescence channel crosstalk.

Easy-to-use Software

The TL-988 Software offers an intuitive experience for protocols programming, plate layouts setting and experimental resultsanalysising. The Software includes an unlimited license for multiple users. The available software modules include Absolute quantification, Melting curve analysis, Relative quantification and High Resolution Melting (HRM). All the excellent features make the instrument to your ideal choice for qPCR.

>> Friendly interface and clearly functional modules design,first-time users can easily start a new experiment.

>> All experiment data and setting options are shown conveniently on a single interface. User modifications to analysis parameters can be seen instantly

>> Powerful analytical capabilities, optional automatic and manual threshold setting bring reliable date you can work on.

>> Experimental date can be export in .csv, .excel, .txt format, with this capability, the TL-988 can interface with your laboratory information system directly.

>> Unique designed universal report can be customized according to the requirements, report review and print function help clinical documentation as needed.

Generate the quality data that you can depend on

Excellent amplification and homogeneity of reaction products

This real-time PCR run was done using 5 ng of hepatitis B virus DNA in each of the 96 block positions. (A) A 135 bp amplicon of the hepatitis B virus gene was amplified using SYBR Green I detection. (B) The reaction product was also subjected to melting curve analysis. In summary, low variation Cq values (Cq range = 0.15and SD = 0.040) and overlapping melt curves of the amplicon (T_mrange of 0.5°C and SD = 0.065) in each of the 96 positions demonstrated temperature homogeneity and equal treatment of all samples - independent of block position.

Dynamic range of gene quantification at low dilutions

A hepatitis B virus gene fragment was amplified in ten serial 1:5 dilutions ranging from 10⁸ to 10⁰ copies per well. Eight replicates were run for each dilution. Results on the TL-988 System show excellent reproducibility and resolution down to very low copy numbers.

Specifications

Thermal Block                                             

Block formats           96-well

Sample volume          5–50 μL

Consumables   0.1ml 96-well unskirted Plates; 0.1ml 8-strip tubes (Optical flat cap)

Max heating/cooling rate          4.2°C/sec

Temperature range        4.0–99.0°C

Temperature accuracy      ≤±0.1℃

Temperature uniformity     ≤ ±0.3°C

Temperature steps          1-99

Heated lid                                                  

Temperature range    100–120°C

Control              Automatic temperature and pressure setting

Optics                                                     

Excitation          4 LEDs

Excitation range     470–630 nm

Detector           Photodiode

Detection range     525–670 nm

Detection channels:

Channel          Excitation (nm)     Emission (nm)      Pre-Calibrated Dyes

1.                 470               525             FAM, SYBR Green I

2.                 523               564             VIC, HEX, TET, JOE

3.                 571               621              ROX, TEXAS-RED

4.                 630               670              CY5

Multiplex            Up to 4 targets

Detection range       10¹-10¹⁰copies

Software                                                    

Analysis modes:   Relative quantification,

                Melt curve analysis, High Resolution Melting

Performance                                                

Reproducibility                                   CV≤2.1%

Correlation coefficient of nucleic acid precision         R≥0.997

System                                                    

Operating systems       Windows XP

Communication         Ethernet or USB

Power usage           AC220 × (1±10%)V, 50 × (1±2%)Hz

Dimensions            540W×300D×480H mm

Weight               35kg