Numerical aperture (NA)

0.1

0.3

0.45

0.65

0.85

Working distance (WD) [mm]

23

18

Glass: 8.93 - 8.18 
  Silicone: 8.93 - 8.18

Glass: 5.09 - 4.35 
  Silicone: 5.09 - 4.79

Glass: 1.48 - 1.18 
  Silicone: 1.48 - 1.33

Objective Field Number

22

22

22

22

22

Immersion medium

Air/Dry

Air/Dry

Air/Dry

Air/Dry

Air/Dry

Spring loaded

N/A

N/A

N/A

N/A

N/A

Correction collar

N/A

N/A

Yes

Yes

Yes

Correction range of correction collar

N/A

N/A

Glass: 0 - 1.2mm 
  Silicone: 0 - 1.2mm

Glass: 0 - 1.2mm 
  Silicone: 0 - 1.2mm

Glass: 0 - 0.7mm 
  Silicone: 0 - 1mm

Iris

N/A

N/A

N/A

N/A

N/A

Correction level of chromatic aberration

-

-

-

-

-

Parfocalizing distance [mm]

45

45

45

45

45

Back focal plane (BFP) position

2.4

-1.2

-3.0

-2.9

-3.1

Type of screw thread

W20.32X0.706(RMS)

W20.32X0.706(RMS)

W20.32X0.706(RMS)

W20.32X0.706(RMS)

W20.32X0.706(RMS)

Brightfield(Reflected)

Limitation

Limitation

Limitation

Limitation

Limitation

Brightfield(Transmitted)

Limitation

Limitation

Limitation

Limitation

Limitation

Darkfield(Reflected)

N/A

N/A

N/A

N/A

N/A

Darkfield(Transmitted)

N/A

N/A

N/A

N/A

N/A

DIC(Reflected)

N/A

N/A

N/A

N/A

N/A

DIC(Transmitted)

N/A

N/A

N/A

N/A

N/A

Phase Contrast

N/A

N/A

N/A

N/A

N/A

Relief Contrast

N/A

N/A

N/A

N/A

N/A

Polarized Light

N/A

N/A

N/A

N/A

Yes

Fluorescence (B, G Excitation)

N/A

N/A

N/A

N/A

N/A

UV Fluorescence (at 365nm)

N/A

N/A

N/A

N/A

N/A

Multiphoton

N/A

N/A

N/A

N/A

N/A

TIRF

N/A

N/A

N/A

N/A

N/A

IR

Yes

Yes

Yes

Yes

Yes

WLI

N/A

N/A

N/A

N/A

N/A

Auto Focus

N/A

N/A

N/A

N/A

N/A