Specifications

Quicking T-2 Toxin Rapid Test is a competitive immunoassay for the semi-quantitative detection of T-2 Toxin

 Introduction

 

Specifications

 Principle: Competitive lateral flow immunochromatographic assay

 Intended use: Detection of T-2 Toxin

 Specimen: Feed or grain                  

 Detection limit: 100 ppb

 Reading time: 5 - 10 min

 Shelf life: 18 months

 Storage: 2- 30 °C. No freezing.

 Packing size: 10 tests / box

  

Advantages

l  Quicking T-2 Toxin Rapid Test enables the detection limit of 100 ppb.

l Quicking T-2 Toxin Rapid Test is a quick and easy testing tool that can be performed in just 10 minutes.

l Quicking T-2 Toxin Rapid Test can be stored for 18 months at room temperature, between 2 and 30°C.

 

Instructions

Kit component  

-      10×foil pouches each contains one T-2 cassette with a pipette and a desiccant

-      2×assay diluent (Diluent A, 30 mL, Diluent B, 8 mL)

-      2×centrifugal tubes (15 mL)

-      Products Manual

 

Test procedure

-          Grind a representative sample to the particle size of fine instant coffee (50% passes through a 20 mesh screen). Weigh out 2.0 g ground portion of the sample into the centrifugal tube (15 mL).

-          Add 2 mL of Diluent A and 8 mL of ethyl acetate into the tube. Shake for 5 min. (If emulsification appears, do a centrifugation at 4000 rpm for 1 min.)

-          Collect accurately 2 mL of supernatant (ethyl acetate layer) into a small container (beaker or tube). Dry the liquid by blowing wind.

-          Redissolve the residues in the small beaker with an amount of the provided Diluent B. (REF: Table )

Cut-off (ppb)	100	200	500	1000
Diluent B (mL)	0.4	0.8	2.0	4.0

-          Take out the cassette from the foil pouch and place it horizontally.

-          Suck the liquid and drop 3 drops into the assay sample hole “S”.

Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.

 

Interpretation of results

Positive: Only one clear band in C zone indicates a positive result. Positive shows that the concentration of T-2 Toxin is at or above 100 ppb in the samples.

Negative: The presence of both bands in C zone and T zone.

Invalid: No band appears in C zone.

 

 

Food Safety Inspection Rapid Test Kit
Myxotoxin Test	Aflatoxin B1	Zearalenone	Vomitoxin
	Ochratoxin A	Fumonisin B1	T-2 toxin
Beta-Agonist Test	Clenbuterol	Ractopamine	Salbutamol
Antibiotics Test	Chloramphenicol	Penicillin	Streptomycin
	Cephapirin	Ceftiofur	Gentamicin
	Tetracyclines	Neomycin	Quinolones
	Sulfadimethoxine	Tylosin
Food Pathogen Test	li O157	Salmonella	Listeria
Other Test	Melamine

  

Food Safety ELISA Kit
Myxotoxin ELISA	Aflatoxin B1	Zearalenone
	Ochratoxin A	Fumonisin B1
Veterinary Drug	Clenbuterol	Ractopamine
Residue ELISA	β- agonists	DES