Product Details

Specifications

1.Competitive elisa to detect Hepatitis B core antibody
2.Used for diagnosis of HBV infection
3.Medical diagnostic reagent

Certificate

GMP

SFDA

ISO13485

ISO9001:2008

HBcAb ELISA kit

CatalogNo. NAME PACKAGE SIZE:

BE105A HBcAb ELISA kit 96 tests/Kit

SUMMARY

This kit is based on solid phase, one step incubation competitive principle ELISA method. Anti HBc if present in the sample, compete with monoclonal anti-HBc conjugated to horseradish peroxidase (HRPConjugate) for a fixed amount of purified HBcAg pre-coated in the wells. When no anti-HBc presents in the sample, the HRP-conjugated anti-HBc will be bound with the antigens inside the wells and any unbound HRP-Conjugate is removed during washing. Chromogen A and B solutions are added into the wells and during incubation, the colorless Chromogens are hydrolyzed by the bound HRP-Conjugate to a blue-colored product. The blue color turns yellow after stopping the reaction with sulfuric acid. No or low color developing suggests for presence of antibodies to HBcAg in the sample.
PRINCIPLE

This kit is an enzyme-linked immunosorbent assay for qualitative detection of antibodies to hepatitis B virus core antigen (anti-HBc) in human serum or plasma. It is intended for use in clinical laboratories for diagnosis and management of patients related to infection with hepatitis B virus. ANTIBODIES TO HEPATITIS B VIRUS CORE ANTIGEN ELISA Kit. One-Step Incubation, Competition Principle

MATERIALS REQUIRED BUT NOT PROVIDED

1. Micropipettes: 0.02, 0.05, 0.10, 0.15, 0.20, and 1.0 ml.

2. Disposable pipette tips.

3. Distilled or deionized water.

4. Humidified Box capable of maintaining 37°C

5. Absorbent paper or paper towel.

6. Micro titer plate or strip-well washer

7. Micro titer plate reader with 450nm (or 450 nm/630 nm) wavelength

8. Timer

SAMPLE COLLECTION AND PRESERVATION

Blood serum samples are routinely prepared form vein. Blood plasma samples are routinely prepared with routine amount of anticoagulant such as heparin or sodium citrate. Sample can be stored at 4°C if tested within two days. Specimens not required within 3 days should be stored at -20°C. Avoid hemolysis and repetitive freeze and thaw of samples. Samples with cloud or precipitation should be centrifugated or filtered before test. Prevent serum from bacterium contamination during collecting and storing.

1 block (96wells)

1 vial(0.5ml)

1 vial (0.5ml)

1 bottle(6ml)

1 bottle (6ml)

1 bottle (20ml)

1 bag

3 pieces

1 each

1. Coated Plate

2. Negative Control

3. Positive Control