UV-6300PC DOUBLE BEAM SPECTROPHOTOMETER
UV-6300PC is an advanced double beam design, with 1.0nm fixed bandwidth ,the two detectors measure sample and reference respectively and simultaneously for optimizing measurement accuracy.They provide excellent performance for measurements in the range of 190 to 1100nm, the memory is 32K. They are suitable for pharmaceutical, biochemical and clinical lab applications as well as routine applications such as quantitative analysis, kinetics, wavelength scan, multiple components and DNA/Protein, PC Windows application software make these instruments versatile. All instruments provide excellent performance for measurements.
Specifications
Model | UV-6300PC |
Wavelength Range | 190-1100nm |
Spectral Bandwidth | 1nm |
Optical System | Double Beam,Grating 1200 lines/mm |
Wavelength Accuracy | ±0.3nm |
Wavelength Repeatability | 0.2nm |
Scan Speed | Hi,MED.,LOW., MAX.3000nm/min |
Photometric Accuray | ±0.3%T or ±0.003A@1A |
Photometric Range | 0-200%T,-0.3-3A |
Stray Light | 0.04%T@220nm,360nm |
Stability | 0.0003A/h@500nm |
Display | 5 inches LCD (320*240 dots) |
Baseline Flatness | ±0.0005A |
Standard Cell Holder | Standard 10mm single cell holder (2pcs) |
Sample Compartment | Standard 10mm pathlength cuvette |
Light Source | Tungsten & Deuterium lamp (pre-aligned) |
Output | USB Type A port for USB memory device(Right side) |
USB Type B port for optional computer connectivity (Back) | |
Parallel Port for Printer | |
Power Requirement | AC 110/220V 50/60HZ |
Dimension(L*W*H) | 589*428*200mm |
Weight | 22kg |
Features
1. Fixed bandwidths.
2. Sealed, solvent-resistant tactile keypad with alpha-numeric entry for file names and units.
3. Pre-aligned deuterium lamp for easy lamp replacement. The status of the lamps may be monitored
4. Powerful built-in program based software UV/Vis Analyst including sophisticated utility programs.
5. Data Download-to-PC software for stand-alone models (Optional)
6. Real-time clock for date and time stamping of results.
7. Data can be saved by USB memory device directly.
Brief Function Introduction
All methods are included as built-in standard; this eliminates the need of software. Online software update via internet.
The local control software includes functions such as: Photometry, Quantitative, Wavelength Scan, Kinetics,DNA/Protein, Multi-wavelength and System Utilities.
Standard Curve
Up to 10 standard solutions may be used to establish calibration equation curve. There is a choice of four methods for fitting a curve through the calibration points: Linear fit, Linear fit through zero, square fit and cubic fit.
Multi-Wavelength
Up to 10 wavelengths may be entered, allowing the measurement of multiple wavelengths on a series of samples.
Wavelength Scan
The Wavelength Scan intervals are 0.1, 0.2, 0.5, 1, 2, 5nm, and High, Medium and Low scan speeds are available. Scan speeds vary from 100 to 2000 nm/min. Wavelengths are scanned from high to low so that the instrument stand by at high wavelength. This minimizes the degradation of UV sensitive samples. Precise control of filter and lamp changes means that their effects are not seen on the final scan. Post-run manipulation includes re-scaling axes, curve tracking and peak picking.
Kinetics
This mode may be used for scanning time course or reacting rate calculations. Abs. vs. time graphs is displayed on the screen in real time.
Wait time and measurement time up to 12 hours may be entered with time intervals of 0.5, 1, 2,5, 10, 30, seconds and 1 min.
Post-run manipulation includes re-scaling, curve tracking and selection of the part of the curve required for the rate calculation. Rate is calculated using a linear regression algorithm before multiplying by the entered factor.
DNA/Protein Test
Concentration and DNA purity are calculated by
Absorbance ratios 260nm/280nm or 260nm/230nm with optional subtracted absorbance at 320nm
DNA Concentration=62.9*A260-36.0*A280
Or 49.1*A260-3.48*A230
Protein Concentration=1552*A260-757.3*A280
Or 183*A260-75.8*A230
Other wavelengths and factors may be entered.
UV Analyst Software (PC-Control software)
The PC application software UV/Vis Analyst takes the best features of the stand-alone version plus more powerful data processing, expanded data collecting, and storage capability. It comes standard with UV3/6 series PC models and is optional to stand-alone models.
The PC application software offers
1. Photometric Mode
2. Quantitative test (Standard curve)
3. Wavelength Scan
4. Kinetics
5. DNA/Protein
6. Multi-Wavelength
7. System Utility
Quantitative Test (Standard curve)
Use up to 20 standards to establish standard curve. Four methods for fitting a curve:
1. Linear fit
2. Linear through zero
3. Square fit
4. Cubic fit
Multi-wavelength
Up to 20 wavelengths can be selected and multiple samples can be measured. (Auto cell changer is required to run multiple samples automatically)
Wavelength Scan
Automatically record peaks and valleys. The quantity of channels is unlimited; you can simultaneously store as many as desired. Post-run manipulation and processing includes:
1. Re-scaling axes, curve.
2. 1st to 4th derivative.
3. Smoothing, combination, zooming, overlap.
Kinetics (Abs. VS Time)
The Kinetics mode may be used for scanning time course or reacting rate calculations. Abs. VS Time graphs are displayed on the screen in real time. Wait time, measurement time and time intervals may be entered.
Post-run manipulation includes re-scaling, curve tracking and selection of the part of the curve required for the rate calculation. Rate is calculated using a linear regression algorithm before multiplying by the entered factor.
DNA/Protein
Concentration and DNA purity are quickly and easily calculated:
Absorbance ratios 260nm/280nm with optional subtracted absorbance at 320nm.
DNA Concentration=62.9*A260-36.0*A280
Protein Concentration=1552*A260-757.3*A280
Other wavelengths and factors may be entered.