Aflatoxin B1 (AFB1) ELISA Test Kit
Storage Conditions
l The kit shall be stored at [2-8 ℃]. Expiry date: 12 months
l The opened Microelisa Stripplate can be stored at [2-8 ℃] and avoid damp. Use for at least 2 months.
IntroductionThe test kit is detecting for the quantitative analysis of Aflatoxin B1 in cereals, fodder, and other sample.
Reagent | Quantity |
Microelisa Stripplate | 8 well× 12strips |
Standard: 0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, 1.62ppb (black cap) | 1×1.0ml |
High standard 100ppb (black cap) | 1×1.0ml |
Antibody working solution (blue cap) | 1×5.5ml |
Enzyme conjugate (red cap) | 1×5.5ml |
Substrate A solution (white cap) | 1×6ml |
Substrate B solution (black cap) | 1×6ml |
Stop Solution (yellow cap) | 1×6ml |
concentrated Wash Solution 20× (white cap) | 1×40ml |
Instructions | 1 |
Adhesive Membrane | 1 |
Sealed bag | 1 |
The Aflatoxin B1 (AFB1) ELISA Test Kit is based on a competitive colorimetric ELISA assay.
The toxin of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target toxin. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the toxin attached to the well. The second antibody, tagged with a peroxidase enzyme, targets the primary antibody that is complexed to the toxin coated on the plate wells, last take coloration with TMB substrate. The samples absorbance value is an inverse relationship with AFB1 content. Lastly, compared with the standard curve can be concluded the AFB1 residues in sample.
Technique Datal Kit sensitivity: 0.02ppb(ng/ml)
l reactive mode: 25℃, 30min～15min
l Detection Limits:
Sample | Detection Limits |
Cereals | 0.1 ppb |
Compound feed. | 0.2 ppb |
Edible oil, peanut | 0.4 ppb |
Sauces, Wheat, Other feed | 0.2 ppb |
Beer | 0.2 ppb |
Wine, soy, vinegar | 0.1 ppb |