Fumonisins B1 (FB1) ELISA Test Kit
l The kit shall be stored at [2-8 ℃]. Expiry date: 12 months
l The opened Microelisa Stripplate can be stored at [2-8 ℃] and avoid damp. Use for at least 2 months.
IntroductionThe test kit is detecting for the quantitative analysis of Fumonisins B1 in corn, feed.
Principle of TestThe Fumonisins B1 (FB1) ELISA Test Kit is based on a competitive colorimetric ELISA assay.
The toxin of interest has been coated in the plate wells. During the analysis, sample is added along with the primary antibody specific for the target toxin and enzyme-conjugate. If the target is present in the sample, it will compete for the antibody, thereby preventing the antibody from binding to the toxin attached to the well. The enzyme-conjugate, will bined with the primary antibody that is complexed to the toxin coated on the plate wells, last, take caloration with TMB substrate. The samples absorbance value is an inverse relationship with FB1 content. Lastly, compared with the standard curve can be concluded the FB1 residues in sample.
Technique Data
l Kit sensitivity: 0.5ppb (ng/ml)
l reactive mode: 25℃, 30min～15min
l Detection Limits:
Sample | Detection Limits |
Corn | 50 ppb |
Feed | 50 ppb |
l Cross-reaction rate:
Fumonisins B1 ………………………….100%
l Sample Recovery rate:
Corn …………………………………..……. 100±15%
Feed …………………………………….. 95±15%