aflatoxin b1 quick test(AFB1 test/Aflatoxin test/aflatoxin b1 quick test/lateral flow immunoassay /ISO9001/ISO1345 certified)

aflatoxin b1 quick test Specifications

Principle: competitive lateral flow immunochromatographic assay

Intended use: Detection of Total Aflatoxin

Specimen: Feed, grain, nuts

Detection limit:5 ppb

Reading time: 5 - 10 min

Shelf life: 18 months

Storage: 2- 30 °C. No freezing.

Packing size: 10 tests / box

Advantages

lQuicking Total aflatoxin b1 quick test  enables the detection limit of 5 ppb.

lQuicking Total aflatoxin b1 quick test  is a quick and easy testing tool that can be performed in just 10 minutes.

lQuicking Total aflatoxin b1 quick test can be stored for 18 months at room temperature, between 2 and 30°C.

Instructions

Kit component

-      10×foil pouches, each containing one TAF cassette with a pipette and a desiccant

-      1×assay diluent (50 mL)

-      2×centrifugal tubes (15 mL)

-      Products Manual

Test procedure

-          Grind a representative sample to the particle size of fine instant coffee (50% passes through a 20 mesh screen). Weigh out 2.0 g ground portion of the sample into the centrifugal tube (15 mL).

-          Add 2 mL of water and 8 mL of ethyl acetate into the tube. Shake for 5 min. (If emulsification appears, do a centrifugation at 4000 rpm for 1 min.)

-          Collect accurately 2 mL of supernatant (ethyl acetate layer) into a small container (beaker or tube). Dry the liquid by blowing wind.

-          Redissolve the residues in the small beaker with an amount of the provided assay diluent. (REF: Table )

-          Take out the cassette from the foil pouch and place it horizontally.

-          Suck the liquid and drip 3 drops into the assay sample hole.

-          Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.

Interpretation of results

Positive: Only one clear band in C zone indicates a positive result. Positive shows that the concentration of Aflatoxin is at or above 10 ppb in the samples.

Negative: The presence of both bands in C zone and T zone.

Invalid: No band appears in C zone.